There is one direct bus a day from Florenc bus station in Prague to Třeboň (138Kč, two hours 50 minutes). Hourly buses run from České Budějovice (31Kč, 30 minutes). The bus station is a 10-minute walk from the centre.
Car & Motorcycle
Třeboň is a two-hour drive south of Prague, starting out south towards Brno on the D1 motorway and then turning off at the E55 Hwy and following the signs. From České Budějovice the drive takes 30 minutes on Hwy 34.
There are six or seven services daily from Prague's main train station to Třeboň (211Kč, 2¾ hours); all require a change of train at Veselí nad Lužnicí.